14ZT

Crystal Structure of FtsZ with Z1143279263

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.627, 88.627, 179.513
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.059 - 1.957
Rwork	0.181
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.745
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.022	1.991
High resolution limit [Å]	1.957	1.957
Rmerge	0.197	2.175
Rmeas	0.205	2.262
Rpim	0.056	0.614
Total number of observations	763658	38044
Number of reflections	57318	2847
<I/σ(I)>	10.7
Completeness [%]	100.0
Redundancy	13.3	13.4
CC(1/2)	0.998	0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-922	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.