14ZS

Crystal Structure of FtsZ with Z1216861874

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.394, 88.394, 179.199
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.236 - 2.435
Rwork	0.177
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.605
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.197	2.477
High resolution limit [Å]	2.435	2.435
Rmerge	0.455	2.830
Rmeas	0.472	2.943
Rpim	0.128	0.804
Total number of observations	401762	19806
Number of reflections	29624	1480
<I/σ(I)>	6.8
Completeness [%]	100.0
Redundancy	13.6	13.4
CC(1/2)	0.986	0.386

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-915	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.