14ZR

Crystal Structure of FtsZ with Z954606858

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	89.171, 89.171, 182.896
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.625 - 2.298
Rwork	0.202
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.616
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.585	2.337
High resolution limit [Å]	2.298	2.298
Rmerge	0.314	1.564
Rmeas	0.327	1.634
Rpim	0.090	0.470
Total number of observations	480573	21736
Number of reflections	36561	1821
<I/σ(I)>	6.8
Completeness [%]	100.0
Redundancy	13.1	11.9
CC(1/2)	0.991	0.383

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-909	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.