14ZQ

Crystal Structure of FtsZ with Z68404778

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.919, 88.919, 180.755
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.499 - 1.961
Rwork	0.190
R-free	0.22480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.679
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.459	1.995
High resolution limit [Å]	1.961	1.961
Rmerge	0.204	2.038
Rmeas	0.213	2.119
Rpim	0.058	0.579
Total number of observations	768593	38527
Number of reflections	57749	2888
<I/σ(I)>	6.1
Completeness [%]	100.0
Redundancy	13.3	13.3
CC(1/2)	0.998	0.328

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-908	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.