14ZO

Crystal Structure of FtsZ with Z1134990241

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	87.716, 87.716, 176.134
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.897 - 2.397
Rwork	0.175
R-free	0.23770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.635
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.858	2.439
High resolution limit [Å]	2.397	2.397
Rmerge	0.314	2.114
Rmeas	0.326	2.205
Rpim	0.089	0.626
Total number of observations	405412	18843
Number of reflections	30038	1517
<I/σ(I)>	7.7
Completeness [%]	100.0
Redundancy	13.5	12.4
CC(1/2)	0.993	0.344

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-794	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.