14ZL

Crystal Structure of FtsZ with Z223388508

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.118, 88.118, 177.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.098 - 2.253
Rwork	0.180
R-free	0.22920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.572
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.059	2.292
High resolution limit [Å]	2.253	2.253
Rmerge	0.344	2.579
Rmeas	0.357	2.680
Rpim	0.097	0.726
Total number of observations	493519	24118
Number of reflections	36482	1785
<I/σ(I)>	7.6
Completeness [%]	99.4
Redundancy	13.5	13.5
CC(1/2)	0.992	0.346

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-776	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.