14ZJ

Crystal Structure of FtsZ with Z396117078

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.585, 88.585, 178.668
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.332 - 1.865
Rwork	0.182
R-free	0.21460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.745
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.292	1.898
High resolution limit [Å]	1.865	1.865
Rmerge	0.217	2.793
Rmeas	0.226	2.903
Rpim	0.062	0.790
Total number of observations	877026	44237
Number of reflections	65805	3281
<I/σ(I)>	10
Completeness [%]	100.0
Redundancy	13.3	13.5
CC(1/2)	0.998	0.373

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-765	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.