14ZG

Crystal Structure of FtsZ with Z119990900

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.247, 88.247, 176.981
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.162 - 2.343
Rwork	0.170
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.523
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.124	2.384
High resolution limit [Å]	2.343	2.343
Rmerge	0.303	1.901
Rmeas	0.315	1.981
Rpim	0.085	0.552
Total number of observations	440253	21194
Number of reflections	32718	1676
<I/σ(I)>	7.8
Completeness [%]	100.0
Redundancy	13.5	12.6
CC(1/2)	0.992	0.364

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-754	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.