14ZF

Crystal Structure of FtsZ with Z57260539

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.621, 88.621, 178.206
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.038 - 2.246
Rwork	0.187
R-free	0.23060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.543
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.001	2.284
High resolution limit [Å]	2.246	2.246
Rmerge	0.303	2.308
Rmeas	0.315	2.404
Rpim	0.087	0.666
Total number of observations	502340	24645
Number of reflections	37713	1909
<I/σ(I)>	5.8
Completeness [%]	100.0
Redundancy	13.3	12.9
CC(1/2)	0.994	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-751	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.