14ZE

Crystal Structure of FtsZ with F6616-6210

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920194
Spacegroup name	P 65
Unit cell lengths	88.649, 88.649, 179.549
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.364 - 1.735
Rwork	0.181
R-free	0.20640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.796
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.325	1.765
High resolution limit [Å]	1.735	1.735
Rmerge	0.132	2.741
Rmeas	0.137	2.847
Rpim	0.037	0.767
Total number of observations	1102793	56132
Number of reflections	82221	4106
<I/σ(I)>	12.9
Completeness [%]	100.0
Redundancy	13.4	13.7
CC(1/2)	0.999	0.331

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-75	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.