14ZD

Crystal Structure of FtsZ with Z32367954

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.955, 88.955, 181.188
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.517 - 2.130
Rwork	0.183
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.658
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.478	2.167
High resolution limit [Å]	2.130	2.130
Rmerge	0.259	2.200
Rmeas	0.269	2.289
Rpim	0.073	0.626
Total number of observations	608135	29598
Number of reflections	45246	2276
<I/σ(I)>	8.9
Completeness [%]	100.0
Redundancy	13.4	13
CC(1/2)	0.996	0.339

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-748	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.