14ZC

Crystal Structure of FtsZ with Z1116966595

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.292, 88.292, 177.261
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.185 - 2.225
Rwork	0.173
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.752
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.146	2.263
High resolution limit [Å]	2.225	2.225
Rmerge	0.294	2.502
Rmeas	0.306	2.600
Rpim	0.083	0.709
Total number of observations	514426	25507
Number of reflections	38289	1891
<I/σ(I)>	8.9
Completeness [%]	100.0
Redundancy	13.4	13.5
CC(1/2)	0.995	0.338

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-742	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.