14ZB

Crystal Structure of FtsZ with Z89307993

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.837, 88.837, 179.388
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.154 - 2.049
Rwork	0.182
R-free	0.21690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.674
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.116	2.084
High resolution limit [Å]	2.049	2.049
Rmerge	0.249	2.357
Rmeas	0.259	2.455
Rpim	0.071	0.682
Total number of observations	668424	32382
Number of reflections	50212	2534
<I/σ(I)>	8.8
Completeness [%]	100.0
Redundancy	13.3	12.8
CC(1/2)	0.996	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-741	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.