14YY

Crystal Structure of FtsZ with Z1220452176

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.306, 88.306, 178.684
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.192 - 2.140
Rwork	0.179
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.588
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.153	2.177
High resolution limit [Å]	2.140	2.140
Rmerge	0.269	2.220
Rmeas	0.280	2.308
Rpim	0.077	0.627
Total number of observations	558110	29164
Number of reflections	42727	2178
<I/σ(I)>	7.8
Completeness [%]	98.5
Redundancy	13.1	13.4
CC(1/2)	0.995	0.344

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-721	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.