14YX

Crystal Structure of FtsZ with Z384468096

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.176, 88.176, 177.427
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.127 - 2.345
Rwork	0.168
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.791
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.088	2.386
High resolution limit [Å]	2.345	2.345
Rmerge	0.321	2.805
Rmeas	0.333	2.922
Rpim	0.091	0.817
Total number of observations	440596	21245
Number of reflections	32662	1665
<I/σ(I)>	7.2
Completeness [%]	100.0
Redundancy	13.5	12.8
CC(1/2)	0.993	0.343

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-718	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.