14YW

Crystal Structure of FtsZ with Z57329001

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.193, 88.193, 177.337
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.135 - 2.024
Rwork	0.179
R-free	0.21820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.678
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.097	2.059
High resolution limit [Å]	2.024	2.024
Rmerge	0.218	2.263
Rmeas	0.227	2.356
Rpim	0.062	0.650
Total number of observations	676736	32948
Number of reflections	50752	2530
<I/σ(I)>	10.1
Completeness [%]	100.0
Redundancy	13.3	13
CC(1/2)	0.997	0.344

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-704	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.