14YV

Crystal Structure of FtsZ with Z1456069604

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.133, 88.133, 177.024
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.798 - 2.030
Rwork	0.181
R-free	0.21720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.677
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.762	2.065
High resolution limit [Å]	2.030	2.030
Rmerge	0.271	2.188
Rmeas	0.282	2.281
Rpim	0.077	0.639
Total number of observations	668741	32622
Number of reflections	50153	2521
<I/σ(I)>	8.4
Completeness [%]	100.0
Redundancy	13.3	12.9
CC(1/2)	0.994	0.342

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-703	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.