14YU

Crystal Structure of FtsZ with Z18839017

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	87.938, 87.938, 177.126
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.008 - 2.249
Rwork	0.170
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.831
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.969	2.287
High resolution limit [Å]	2.249	2.249
Rmerge	0.280	2.572
Rmeas	0.292	2.674
Rpim	0.080	0.730
Total number of observations	494694	24337
Number of reflections	36758	1811
<I/σ(I)>	7.5
Completeness [%]	100.0
Redundancy	13.5	13.4
CC(1/2)	0.995	0.361

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-698	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.