14YT

Crystal Structure of FtsZ with Z26365442

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.327, 88.327, 178.658
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.910 - 2.808
Rwork	0.173
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.534
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.873	2.857
High resolution limit [Å]	2.808	2.808
Rmerge	0.484	2.201
Rmeas	0.505	2.304
Rpim	0.142	0.659
Total number of observations	245946	11142
Number of reflections	19241	986
<I/σ(I)>	5.7
Completeness [%]	100.0
Redundancy	12.8	11.3
CC(1/2)	0.974	0.336

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-691	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.