14YQ

Crystal Structure of FtsZ with Z959357244

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.476, 88.476, 178.771
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.277 - 1.829
Rwork	0.180
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.806
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.238	1.860
High resolution limit [Å]	1.829	1.829
Rmerge	0.157	2.785
Rmeas	0.163	2.894
Rpim	0.045	0.783
Total number of observations	933248	46443
Number of reflections	69693	3417
<I/σ(I)>	11.1
Completeness [%]	100.0
Redundancy	13.4	13.6
CC(1/2)	0.999	0.331

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-684	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.