14YP

Crystal Structure of FtsZ with Z1267800292

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	P 65
Unit cell lengths	88.264, 88.264, 177.409
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.171 - 2.102
Rwork	0.175
R-free	0.21930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.617
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.132	2.139
High resolution limit [Å]	2.102	2.102
Rmerge	0.278	2.471
Rmeas	0.289	2.576
Rpim	0.078	0.724
Total number of observations	606904	27642
Number of reflections	45345	2251
<I/σ(I)>	8.3
Completeness [%]	100.0
Redundancy	13.4	12.3
CC(1/2)	0.995	0.345

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-674	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.