14YN

Crystal Structure of FtsZ with Z31478538

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	P 65
Unit cell lengths	88.740, 88.740, 178.403
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.409 - 1.858
Rwork	0.182
R-free	0.20850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.789
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.370	1.890
High resolution limit [Å]	1.858	1.858
Rmerge	0.211	3.028
Rmeas	0.219	3.148
Rpim	0.060	0.855
Total number of observations	891812	45381
Number of reflections	66706	3351
<I/σ(I)>	9.4
Completeness [%]	100.0
Redundancy	13.4	13.5
CC(1/2)	0.997	0.383

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-656	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.