14YM

Crystal Structure of FtsZ with Z19735981

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.627, 88.627, 178.659
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.353 - 1.702
Rwork	0.182
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.819
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.313	1.732
High resolution limit [Å]	1.702	1.702
Rmerge	0.148	3.072
Rmeas	0.154	3.187
Rpim	0.041	0.845
Total number of observations	1163774	58802
Number of reflections	86590	4304
<I/σ(I)>	11.2
Completeness [%]	100.0
Redundancy	13.4	13.7
CC(1/2)	0.999	0.358

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-655	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.