14YL

Crystal Structure of FtsZ with Z18197050

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	P 65
Unit cell lengths	89.091, 89.091, 181.432
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.585 - 2.090
Rwork	0.180
R-free	0.21480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.738
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.546	2.126
High resolution limit [Å]	2.090	2.090
Rmerge	0.149	2.307
Rmeas	0.155	2.408
Rpim	0.042	0.688
Total number of observations	644499	29785
Number of reflections	48108	2448
<I/σ(I)>	13
Completeness [%]	100.0
Redundancy	13.4	12.2
CC(1/2)	0.999	0.368

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-651	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.