14YK

Crystal Structure of FtsZ with Z445191014

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.585, 88.585, 178.919
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.332 - 1.795
Rwork	0.178
R-free	0.20630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.787
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.292	1.826
High resolution limit [Å]	1.795	1.795
Rmerge	0.145	2.411
Rmeas	0.151	2.503
Rpim	0.041	0.668
Total number of observations	989807	50501
Number of reflections	73904	3704
<I/σ(I)>	12
Completeness [%]	100.0
Redundancy	13.4	13.6
CC(1/2)	0.999	0.385

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-647	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.