14YJ

Crystal Structure of FtsZ with Z1400796579

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.572, 88.572, 179.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.036 - 1.929
Rwork	0.179
R-free	0.21190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.785
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.999	1.962
High resolution limit [Å]	1.929	1.929
Rmerge	0.174	2.065
Rmeas	0.180	2.147
Rpim	0.049	0.585
Total number of observations	798112	39938
Number of reflections	59847	2975
<I/σ(I)>	11.2
Completeness [%]	100.0
Redundancy	13.3	13.4
CC(1/2)	0.998	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-643	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.