14YI

Crystal Structure of FtsZ with Z208334100

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.175, 88.175, 177.765
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.126 - 1.925
Rwork	0.179
R-free	0.21490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.711
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.088	1.958
High resolution limit [Å]	1.925	1.925
Rmerge	0.235	2.910
Rmeas	0.245	3.026
Rpim	0.067	0.826
Total number of observations	789358	39969
Number of reflections	59089	2971
<I/σ(I)>	9.4
Completeness [%]	100.0
Redundancy	13.4	13.5
CC(1/2)	0.997	0.343

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-642	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.