14YG

Crystal Structure of FtsZ with Z53825177

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.660, 88.660, 179.398
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.369 - 1.790
Rwork	0.179
R-free	0.20730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.566
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.330	1.820
High resolution limit [Å]	1.790	1.790
Rmerge	0.152	2.991
Rmeas	0.158	3.106
Rpim	0.043	0.834
Total number of observations	1004029	50751
Number of reflections	74912	3716
<I/σ(I)>	11.4
Completeness [%]	100.0
Redundancy	13.4	13.7
CC(1/2)	0.999	0.339

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-636	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.