14YF

Crystal Structure of FtsZ with Z300245038

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920051
Spacegroup name	P 65
Unit cell lengths	88.456, 88.456, 178.578
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.968 - 2.385
Rwork	0.191
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.619
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.931	2.426
High resolution limit [Å]	2.385	2.385
Rmerge	0.359	2.019
Rmeas	0.374	2.118
Rpim	0.103	0.636
Total number of observations	411944	17286
Number of reflections	31438	1574
<I/σ(I)>	6.9
Completeness [%]	100.0
Redundancy	13.1	11
CC(1/2)	0.992	0.343

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-634	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.