14YC

Crystal Structure of FtsZ with Z212122838

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.973, 88.973, 179.598
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.526 - 1.982
Rwork	0.183
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.709
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.486	2.017
High resolution limit [Å]	1.982	1.982
Rmerge	0.177	2.165
Rmeas	0.184	2.251
Rpim	0.050	0.614
Total number of observations	739678	36822
Number of reflections	55623	2786
<I/σ(I)>	10.7
Completeness [%]	100.0
Redundancy	13.3	13.2
CC(1/2)	0.998	0.326

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-611	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.