14YA

Crystal Structure of FtsZ with F2147-0982

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920194
Spacegroup name	P 65
Unit cell lengths	88.780, 88.780, 179.020
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.429 - 1.936
Rwork	0.179
R-free	0.20910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.749
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.390	1.970
High resolution limit [Å]	1.936	1.936
Rmerge	0.184	2.018
Rmeas	0.192	2.098
Rpim	0.053	0.569
Total number of observations	788643	39885
Number of reflections	59231	2979
<I/σ(I)>	10.4
Completeness [%]	100.0
Redundancy	13.3	13.4
CC(1/2)	0.998	0.388

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-59	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.