14XY

Crystal Structure of FtsZ with Z32327641

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.263, 88.263, 177.685
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.170 - 2.017
Rwork	0.181
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.689
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.132	2.052
High resolution limit [Å]	2.017	2.017
Rmerge	0.224	2.395
Rmeas	0.233	2.493
Rpim	0.064	0.690
Total number of observations	684574	33376
Number of reflections	51427	2558
<I/σ(I)>	9.2
Completeness [%]	100.0
Redundancy	13.3	13
CC(1/2)	0.997	0.336

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-573	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.