14XS

Crystal Structure of FtsZ with Z364577298

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.416, 88.416, 178.361
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.247 - 1.930
Rwork	0.180
R-free	0.21420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.690
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.208	1.964
High resolution limit [Å]	1.930	1.930
Rmerge	0.216	2.788
Rmeas	0.224	2.899
Rpim	0.061	0.790
Total number of observations	788969	39864
Number of reflections	59076	2969
<I/σ(I)>	9.6
Completeness [%]	100.0
Redundancy	13.4	13.4
CC(1/2)	0.998	0.352

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-542	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.