14XK

Crystal Structure of FtsZ with Z64450924

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.524, 88.524, 178.077
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.301 - 1.820
Rwork	0.179
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.771
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.262	1.852
High resolution limit [Å]	1.820	1.820
Rmerge	0.164	2.750
Rmeas	0.170	2.854
Rpim	0.046	0.761
Total number of observations	942305	47765
Number of reflections	70485	3514
<I/σ(I)>	11.6
Completeness [%]	100.0
Redundancy	13.4	13.6
CC(1/2)	0.999	0.345

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-513	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.