14XH

Crystal Structure of FtsZ with Z26823525

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.583, 88.583, 178.837
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.030 - 1.992
Rwork	0.177
R-free	0.21050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.709
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.993	2.027
High resolution limit [Å]	1.992	1.992
Rmerge	0.210	2.526
Rmeas	0.218	2.627
Rpim	0.060	0.720
Total number of observations	720579	35611
Number of reflections	54092	2696
<I/σ(I)>	9.8
Completeness [%]	100.0
Redundancy	13.3	13.2
CC(1/2)	0.997	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-507	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.