14WY

Crystal Structure of FtsZ with F9994-0329

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920194
Spacegroup name	P 65
Unit cell lengths	88.523, 88.523, 178.418
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.301 - 2.002
Rwork	0.180
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.683
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.262	2.037
High resolution limit [Å]	2.002	2.002
Rmerge	0.179	1.981
Rmeas	0.186	2.061
Rpim	0.051	0.568
Total number of observations	707193	34801
Number of reflections	53091	2647
<I/σ(I)>	11
Completeness [%]	100.0
Redundancy	13.3	13.1
CC(1/2)	0.998	0.362

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-47	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.