14WW

Crystal Structure of FtsZ with Z1333043510

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.815, 88.815, 179.208
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.447 - 1.951
Rwork	0.179
R-free	0.20840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.608
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.408	1.984
High resolution limit [Å]	1.951	1.951
Rmerge	0.170	2.362
Rmeas	0.177	2.454
Rpim	0.048	0.666
Total number of observations	774145	39389
Number of reflections	58064	2934
<I/σ(I)>	11.2
Completeness [%]	100.0
Redundancy	13.3	13.4
CC(1/2)	0.998	0.343

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-465	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.