14WV

Crystal Structure of FtsZ with Z100642432

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.651, 88.651, 179.205
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.365 - 1.931
Rwork	0.180
R-free	0.21330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.738
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.326	1.964
High resolution limit [Å]	1.931	1.931
Rmerge	0.207	2.575
Rmeas	0.215	2.678
Rpim	0.059	0.732
Total number of observations	794479	39397
Number of reflections	59616	2936
<I/σ(I)>	10.5
Completeness [%]	100.0
Redundancy	13.3	13.4
CC(1/2)	0.998	0.373

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-464	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.