14WR

Crystal Structure of FtsZ with Z285233820

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.642, 88.642, 178.514
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.360 - 1.829
Rwork	0.180
R-free	0.20860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.784
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.321	1.861
High resolution limit [Å]	1.830	1.830
Rmerge	0.162	2.288
Rmeas	0.168	2.378
Rpim	0.046	0.643
Total number of observations	932467	46792
Number of reflections	69761	3451
<I/σ(I)>	12.1
Completeness [%]	100.0
Redundancy	13.4	13.6
CC(1/2)	0.999	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-457	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.