14WQ

Crystal Structure of FtsZ with Z955369596

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.485, 88.485, 179.156
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.989 - 1.867
Rwork	0.182
R-free	0.21190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.748
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.952	1.899
High resolution limit [Å]	1.867	1.867
Rmerge	0.196	2.634
Rmeas	0.204	2.738
Rpim	0.056	0.745
Total number of observations	876895	43818
Number of reflections	65657	3250
<I/σ(I)>	10.3
Completeness [%]	100.0
Redundancy	13.4	13.5
CC(1/2)	0.998	0.383

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-453	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.