14WP

Crystal Structure of FtsZ with Z1220645147

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.399, 88.399, 179.060
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.239 - 2.063
Rwork	0.176
R-free	0.21490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.659
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.200	2.098
High resolution limit [Å]	2.063	2.063
Rmerge	0.269	3.234
Rmeas	0.279	3.369
Rpim	0.076	0.941
Total number of observations	650293	30715
Number of reflections	48611	2428
<I/σ(I)>	8.2
Completeness [%]	100.0
Redundancy	13.4	12.7
CC(1/2)	0.996	0.324

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-450	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.