14WO

Crystal Structure of FtsZ with Z57778470

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.745, 88.745, 179.723
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.116 - 2.057
Rwork	0.179
R-free	0.21160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.681
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.079	2.092
High resolution limit [Å]	2.057	2.057
Rmerge	0.220	2.756
Rmeas	0.229	2.873
Rpim	0.063	0.810
Total number of observations	662629	31180
Number of reflections	49580	2473
<I/σ(I)>	9.6
Completeness [%]	100.0
Redundancy	13.4	12.6
CC(1/2)	0.997	0.354

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-437	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.