14WN

Crystal Structure of FtsZ with F6672-1327

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920194
Spacegroup name	P 65
Unit cell lengths	88.739, 88.739, 178.540
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.409 - 1.906
Rwork	0.181
R-free	0.21390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.714
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.369	1.938
High resolution limit [Å]	1.906	1.906
Rmerge	0.170	2.543
Rmeas	0.177	2.644
Rpim	0.049	0.721
Total number of observations	825390	41815
Number of reflections	61925	3110
<I/σ(I)>	11
Completeness [%]	100.0
Redundancy	13.3	13.4
CC(1/2)	0.999	0.309

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-43	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.