14WM

Crystal Structure of FtsZ with Z57299529

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.903, 88.903, 178.891
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.491 - 2.302
Rwork	0.179
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.595
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.451	2.342
High resolution limit [Å]	2.302	2.302
Rmerge	0.373	3.631
Rmeas	0.388	3.777
Rpim	0.105	1.033
Total number of observations	476921	23594
Number of reflections	35370	1791
<I/σ(I)>	6.9
Completeness [%]	100.0
Redundancy	13.5	13.2
CC(1/2)	0.993	0.339

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-426	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.