14WK

Crystal Structure of FtsZ with Z30857828

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.716, 88.716, 180.515
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.397 - 1.923
Rwork	0.181
R-free	0.21120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.772
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.358	1.956
High resolution limit [Å]	1.923	1.923
Rmerge	0.194	2.795
Rmeas	0.202	2.907
Rpim	0.055	0.795
Total number of observations	812849	40269
Number of reflections	60866	2997
<I/σ(I)>	10.2
Completeness [%]	100.0
Redundancy	13.4	13.4
CC(1/2)	0.998	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-423	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.