14WJ

Crystal Structure of FtsZ with Z31408124

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.676, 88.676, 179.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.377 - 2.049
Rwork	0.179
R-free	0.21110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.647
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.338	2.084
High resolution limit [Å]	2.049	2.049
Rmerge	0.211	2.546
Rmeas	0.219	2.652
Rpim	0.060	0.741
Total number of observations	669455	31893
Number of reflections	50138	2498
<I/σ(I)>	6.7
Completeness [%]	100.0
Redundancy	13.4	12.8
CC(1/2)	0.998	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-421	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.