14WI

Crystal Structure of FtsZ with F6482-3468

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920194
Spacegroup name	P 65
Unit cell lengths	88.676, 88.676, 179.701
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.377 - 2.124
Rwork	0.182
R-free	0.21910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.669
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.338	2.160
High resolution limit [Å]	2.124	2.124
Rmerge	0.238	2.125
Rmeas	0.248	2.214
Rpim	0.068	0.617
Total number of observations	605407	29530
Number of reflections	45021	2278
<I/σ(I)>	8.8
Completeness [%]	100.0
Redundancy	13.4	13
CC(1/2)	0.996	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-42	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.