14WH

Crystal Structure of FtsZ with Z44592329

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.592, 88.592, 178.767
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.335 - 1.843
Rwork	0.181
R-free	0.21030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.768
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.296	1.874
High resolution limit [Å]	1.843	1.843
Rmerge	0.160	2.505
Rmeas	0.166	2.603
Rpim	0.045	0.704
Total number of observations	912763	46063
Number of reflections	68291	3400
<I/σ(I)>	11.3
Completeness [%]	100.0
Redundancy	13.4	13.5
CC(1/2)	0.999	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-419	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.