14WG

Crystal Structure of FtsZ with Z26333434

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.733, 88.733, 179.816
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.406 - 2.060
Rwork	0.177
R-free	0.21730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.787
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.367	2.096
High resolution limit [Å]	2.060	2.060
Rmerge	0.198	2.275
Rmeas	0.206	2.373
Rpim	0.057	0.670
Total number of observations	657307	30754
Number of reflections	49342	2456
<I/σ(I)>	9.4
Completeness [%]	100.0
Redundancy	13.3	12.5
CC(1/2)	0.998	0.347

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-418	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.