14WE

Crystal Structure of FtsZ with Z94597856

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919764
Spacegroup name	P 65
Unit cell lengths	88.781, 88.781, 180.249
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.140 - 2.093
Rwork	0.179
R-free	0.21550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.741
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.103	2.129
High resolution limit [Å]	2.093	2.093
Rmerge	0.185	2.223
Rmeas	0.194	2.355
Rpim	0.061	0.775
Total number of observations	476601	21584
Number of reflections	47239	2349
<I/σ(I)>	9.7
Completeness [%]	100.0
Redundancy	10.1	9.2
CC(1/2)	0.997	0.358

Crystallization Conditions

crystal ID	method	pH	temperature	details
ftsz-410	VAPOR DIFFUSION, SITTING DROP	5	294	0.1 M ammonium sulfate, 15.6% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.0.